首页> 外文期刊>Journal of Clinical Microbiology >Moredun Bordetella Medium, an improved selective medium for isolation of Bordetella parapertussis.
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Moredun Bordetella Medium, an improved selective medium for isolation of Bordetella parapertussis.

机译:Moredun博德特氏菌培养基,一种改良的选择性分离百日咳博德特氏菌的培养基。

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Bordetella parapertussis, previously thought to be an obligate human respiratory tract pathogen, has been isolated from sheep. Attempts to assess the prevalence of B. parapertussis in conventionally reared sheep by nasal swabbing proved futile with existing selective media because of extensive overgrowth with Mucor spp. and other nasal commensals. Moredun Bordetella Medium (MBM), which contains cycloheximide and spectinomycin at final concentrations of 0.5 mg/ml and 100 mu g/ml, respectively, was developed as an improved selective medium to isolate B. parapertussis from the nasal cavities of conventionally reared sheep. The selective ability of MBM was evaluated with 200 nasal swabs from conventionally reared sheep, and B. parapertussis was recovered from 31.5% of the samples. MBM facilitated the simple and effective isolation of B. parapertussis from ovine nasal swabs and, in successfully excluding overgrowth with other contaminants, proved superior to other test formulations evaluated and to existing conventional media.
机译:百日咳博德特氏菌以前被认为是人类呼吸道的病原体,现已从绵羊中分离出来。尝试通过鼻擦拭评估常规饲养的绵羊的百日咳博德特氏菌的患病率对现有的选择性培养基是徒劳的,因为穆克氏菌属植物过度繁殖。和其他鼻腔礼盒已开发出莫瑞登氏博德特氏菌培养基(MBM),其分别包含终浓度为0.5 mg / ml和100μg/ ml的环己酰亚胺和壮观霉素,作为一种改良的选择性培养基,可将百日咳杆菌从常规饲养的羊的鼻腔中分离出来。用常规饲养的200只鼻拭子评估MBM的选择性能力,并从31.5%的样本中回收了百日咳杆菌。 MBM有助于从猪鼻拭子中简单有效地分离出百日咳博德特氏菌,并且成功地排除了其他污染物的过度生长,事实证明,MBM优于评估的其他测试制剂和现有的常规培养基。

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