A Conserved Element in the Protein-Coding Sequence Is Required for Normal Expression of Replication-Dependent Histone Genes in DevelopingXenopusEmbryos ☆

Andrew Ficzycz; Nikola K. Kaludov; Zsolt Lele; Myra M. Hurt; Nick Ovsenek

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A Conserved Element in the Protein-Coding Sequence Is Required for Normal Expression of Replication-Dependent Histone Genes in DevelopingXenopusEmbryos ☆

机译：在非洲爪蟾中，复制依赖性组蛋白基因的正常表达需要蛋白质编码序列中的保守元素☆

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Replication-dependenthistonegenesinthemouseandemXenopus/emshareacommonregulatoryelementwithintheprotein-encodingsequencecalledtheCRASαelement(codingregionactivatingsequenceα)whichhasbeenshowntomediatenormalexpressioneminvivo/emandtointeractwithnuclearfactorseminvitro/eminacellcycle-dependentmanner.Thusfar,theαelementhasonlybeenstudiedinrodentcellsinculture,anditseffectonhistonegeneexpressionduringdevelopmenthasnotbeendetermined.HereweexaminetheroleoftheαelementinhistonegeneexpressionduringemXenopus/emdevelopmentwhichfeaturesaswitchinhistonegeneexpressionfromareplication-independentmodeinoocytestoareplication-dependentmodeinembryosaftermidblastulastage.emInvivo/emexpressionexperimentsinvolvingwild-typeorα-mutantmouseH3.2genesshowthatmutationoftheCRASαelementresultsinafourfolddeclineofexpressioninembryos,butdoesnotaffectexpressioninoocytes.Twodistinctαsequence-specificbindingactivitiesweredetectedinbothoocyteandembryonicextracts.AslowlymigratingDNA-bindingcomplexwaspresentatrelativelyconstantlevelsthroughoutdevelopmentfromtheearlieststagesofoogenesisthroughlarvalstages.Incontrast,levelsofarapidlymigratingcomplexwerehighinstageIandIIoocytes,declinedinstageIIndash;VIoocytes,remainedlowinunfertilizedeggsandcleavagestageembryos,androsedramaticallyafterthemidblastulatransition.Themolecularmassesofthefactorsformingtheslowandrapidlymigratingcomplexeswereestimatedtobeapproximately110and85kDa,respectively.DNA-bindingactivityofthe85kDaα-bindingfactorwasaffectedbyphosphorylation,bindingwithhigheraffinityinthedephosphorylatedstate.TheabruptincreaseinDNA-bindingactivityofthe85-kDaα-bindingfactoratlateblastulacoincideswiththeswitchtothereplication-dependentmodeofhistonegeneexpression.WeproposethattheconservedαelementpresentinthecodingsequenceofmouseandemXenopus/emcorehistonegenesisrequiredfornormalreplication-dependenthistoneexpressioninthedevelopingemXenopus/emembryo./p/div

机译：复制dependenthistonegenesinthemouseand 爪蟾 shareacommonregulatoryelementwithintheprotein-encodingsequencecalledtheCRASαelement（codingregionactivatingsequenceα）whichhasbeenshowntomediatenormalexpression INVIVO andtointeractwithnuclearfactors 体外 inacellcycle-dependentmanner.Thusfar，theαelementhasonlybeenstudiedinrodentcellsinculture，anditseffectonhistonegeneexpressionduringdevelopmenthasnotbeendetermined.Hereweexaminetheroleoftheαelementinhistonegeneexpressionduring非洲爪 developmentwhichfeaturesaswitchinhistonegeneexpressionfromareplication-independentmodeinoocytestoareplication-dependentmodeinembryosaftermidblastulastage。 INVIVO expressionexperimentsinvolvingwild-typeorα-mutantmouseH3.2genesshowthatmutationoftheCRASαelementresultsinafourfolddeclineofexpressioninembryos，butdoesnotaffectexpressioninoocytes.Twodistinctαsequence-specificbindingactivitiesweredetectedinbothoocyteandembryonicextracts.AslowlymigratingDNA-bindingcomplexwaspr esentatrelativelyconstantlevelsthroughoutdevelopmentfromtheearlieststagesofoogenesisthroughlarvalstages.Incontrast，levelsofarapidlymigratingcomplexwerehighinstageIandIIoocytes，declinedinstageIIndash; VIoocytes，remainedlowinunfertilizedeggsandcleavagestageembryos，androsedramaticallyafterthemidblastulatransition.Themolecularmassesofthefactorsformingtheslowandrapidlymigratingcomplexeswereestimatedtobeapproximately110and85kDa，respectively.DNA-bindingactivityofthe85kDaα-bindingfactorwasaffectedbyphosphorylation，bindingwithhigheraffinityinthedephosphorylatedstate.TheabruptincreaseinDNA-bindingactivityofthe85kDaα-bindingfactoratlateblastulacoincideswiththeswitchtothereplication-dependentmodeofhistonegeneexpression.Weproposethattheconservedαelementpresentinthecodingsequenceofmouseand爪蟾 corehistonegenesisrequiredfornormalreplication-dependenthistoneexpressioninthedeveloping 非洲爪蟾胚胎。 展开▼

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《Developmental biology》 |1997年第1期|共页

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Andrew Ficzycz; Nikola K. Kaludov; Zsolt Lele; Myra M. Hurt; Nick Ovsenek;
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中图分类生物科学;

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专利

1. A CONSERVED ELEMENT IN THE PROTEIN-CODING SEQUENCE IS REQUIRED FOR NORMAL EXPRESSION OF REPLICATION-DEPENDENT HISTONE GENES IN DEVELOPING XENOPUS EMBRYOS [J] . Ficzycz A., Lele Z., Hurt MM., Developmental biology . 1997,第1期

机译：蛋白质表达序列中的保守元素对于发育中的非洲人胚胎的复制依赖的组蛋白基因的正常表达是必需的

2. THE CODING SEQUENCES OF MOUSE H2A AND H3 HISTONE GENES CONTAINS A CONSERVED SEVEN NUCLEOTIDE ELEMENT THAT INTERACTS WITH NUCLEAR FACTORS AND IS NECESSARY FOR NORMAL EXPRESSION [J] . Bowman TL, Hurt MM Nucleic Acids Research . 1995,第16期

机译：小鼠H2A和H3组蛋白基因的编码序列包含一个保守的七个核苷酸元素，该元素与核因子相互作用，是正常表达所必需的

3. The coding sequences of mouse H2A and H3 histone genes contains a conserved seven nucleotide element that interacts with nuclear factors and is necessary for normal expression [J] . Myra M. Hurt, Tammy L. Bowman Nucleic acids research . 1995,第16期

机译：小鼠H2A和H3组蛋白基因的编码序列包含一个与核因子相互作用的保守的七个核苷酸元件，对于正常表达是必需的

4. LncRNAs and Protein-coding Genes Expression Analysis for Myelodysplastic Syndromes Diagnoses [C] . Lama Al-Bakhat, Norah Al-Serhani International Conference on Artificial Intelligence Modern Assistive Technology . 2020

机译：髓细胞增强综合征诊断的LNCRNA和蛋白质编码基因表达分析

5. Role for the mammalian transcription factor YY1 and its Drosophila homologue pho in regulation of replication-dependent histone gene expression. [D] . Marley, Katherine Eliassen. 2000

机译：哺乳动物转录因子YY1及其果蝇同系物pho在调节复制依赖性组蛋白基因表达中的作用。

6. The coding sequences of mouse H2A and H3 histone genes contains a conserved seven nucleotide element that interacts with nuclear factors and is necessary for normal expression. [O] . T L Bowman, M M Hurt 1995

机译：小鼠H2A和H3组蛋白基因的编码序列包含一个与核因子相互作用的保守的七个核苷酸元件是正常表达所必需的。

7. A Conserved Element in the Protein-Coding Sequence Is Required for Normal Expression of Replication-Dependent Histone Genes in DevelopingXenopusEmbryos [O] . Ficzycz Andrew, Kaludov Nikola K., Lele Zsolt, 1997

机译：在发展中国家爪蟾复制依赖组蛋白基因的正常表达需要蛋白质编码序列中的保守元素。

1. 淀粉样β蛋白前体转基因小鼠与正常小鼠脑蛋白质组蛋白质表达差异 [J] . 杨国锋 ,彭立威 ,姚建华 . 中国组织工程研究 . 2006,第018期

2. 雌二醇短期暴露对非洲爪蟾性腺和输卵管形态及性二态基因表达影响的初步研究 [J] . 徐伟 ,楼钦钦 ,魏无际 . 生态毒理学报 . 2015,第001期

3. 非洲爪蟾干扰素-λ1基因的原核表达 [J] . 齐志涛 ,高谦 ,张启焕 . 水生生物学报 . 2011,第006期

4. 人类基因组中蛋白质编码序列数目随其长度的分布研究 [J] . 冯立芹 ,李宏 . 内蒙古民族大学学报（自然科学版） . 2009,第001期

5. Fox家族在非洲爪蟾胚胎发育中的表达模式及作用 [J] . 申姗姗 ,任斐 ,陈晓虹 . 广东农业科学 . 2013,第007期

6. 大豆花叶病毒基因组中蛋白质编码序列的克隆与表达 [C] . 张景凤 ,赵慧 . 中国植物病理学会第六届代表大会暨学术年会 . 1998

7. Sideroflexin家族基因在非洲爪蟾胚胎中的表达与功能研究 [A] . 李晓静 . 2010

1. 在丝状真菌细胞中选择性碳源非依赖性表达蛋白质编码序列的方法 [P] . 中国专利： CN109715809A . 2019-05-03

2. 一种提高大肠杆菌中可溶性重组蛋白质表达水平的方法 [P] . 中国专利： CN105087725A . 2015-11-25

3. New variants of the human Tissue Inhibitor Of Metalloproteinase-2 and Matrix Metalloproteinase-2 genes, for diagnosis of pathologically abnormal gene expression, and use of normal gene sequences in gene therapy [P] . 外国专利： DE10241553A1 . 2004-03-18

机译：人类金属蛋白酶2和基质金属蛋白酶2基因组织抑制剂的新变体，用于诊断病理上异常的基因表达，并在基因治疗中使用正常基因序列

4. PLANT GENE EXPRESSION VECTOR FAMILY BASED ON THE REGULATORY DNA SEQUENCES OF AN ALFALFA H3 HISTONE GENE VARIANT (MsH3g1) [P] . 外国专利： WO9720058A2 . 1997-06-05

机译：基于苜蓿H3组蛋白基因变异体（MsH3g1）调控DNA序列的植物基因表达载体家族

5. Method for selective carbon source-dependent expression of protein-coding sequences in a filamentous fungal cell [P] . 外国专利： DK3296401T3 . 2020-05-04

机译：在丝状真菌细胞中选择性依赖碳源表达蛋白质编码序列的方法

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A Conserved Element in the Protein-Coding Sequence Is Required for Normal Expression of Replication-Dependent Histone Genes in DevelopingXenopusEmbryos ☆

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