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Studies of oogenesis in Urechis caupo: II. Accumulation during oogenesis of carbohydrate, RNA, microtubule protein, and soluble, mitochondrial, and lysosomal enzymes☆

机译:Urechis caupo中卵子发生的研究:II。碳水化合物,RNA,微管蛋白以及可溶性,线粒体和溶酶体酶在卵子发生过程中的积累☆

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IntheechiuroidwormUrechiscaupo,theoocytesdevelopindependentlyassinglecellsinthecoelomicfluid.Theaccumulationofoocyteconstituentsduringoogenesiswasdeterminedindifferentsizeclassesofcoelomicoocytes,separatedbyfractionationonaFicolldensitygradient.Eachsizeclasswasassayedforprotein,carbohydrate,RNA,microtubuleprotein,glucose-6-phosphatedehydrogenase,acidphosphatase,andcytochromecoxidase.Alltheconstituentsinvestigatedaccumulatedcontinuouslyduringoogenesis,andtheratesofaccumulationparalleledthevolumeincreaseoftheoocytes.Theelectrophoreticpatternofsolubleeggproteinssupportedthisfinding.Thesedatasuggestthatthegenesinvolvedinthesynthesisofalltheoocyteconstituentsstudiedareactivatedearlyinoogenesis.
机译:IntheechiuroidwormUrechiscaupo,theoocytesdevelopindependentlyassinglecellsinthecoelomicfluid.Theaccumulationofoocyteconstituentsduringoogenesiswasdeterminedindifferentsizeclassesofcoelomicoocytes,separatedbyfractionationonaFicolldensitygradient.Eachsizeclasswasassayedforprotein,碳水化合物,RNA,microtubuleprotein,葡萄糖-6- phosphatedehydrogenase,acidphosphatase,andcytochromecoxidase.Alltheconstituentsinvestigatedaccumulatedcontinuouslyduringoogenesis,andtheratesofaccumulationparalleledthevolumeincreaseoftheoocytes.Theelectrophoreticpatternofsolubleeggproteinssupportedthisfinding.Thesedatasuggestthatthegenesinvolvedinthesynthesisofalltheoocyteconstituentsstudiedareactivatedearlyinoogenesis。

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