Groupes sulfhydriles et morphogénèse: I. Effets du β-mercaptoéthanol, de l'acide α-lipo?que, de l'acide adénosinetriphosphorique et de l'oxaloacétate sur le développement embryonnaire des Batraciens

摘要

1.1.Theeffectsofmercaptoethanol,lipoicacid,ATPandoxaloacetate,aloneorincombination,havebeenstudiedineggsof4differentspeciesofamphibians.2.2.Theeffectsofmercaptoethanol(M100)andlipoicacid(20a€“30??g/ml)areremarkablysimilar:cleavageislittleaffected,butgastrulationandneuralplateclosurearestronglyinhibited.Atlowerconcentrations,outgrowthofthetailisstronglyreduced.3.3.Mercaptoethanolandlipoicacid,ifmixedtogether,haveadditiveeffects.4.4.ATP,whichspeedsupneuralplateclosure,isantagonistictobothmercaptoethanolandlipoicacidsofarasneurulationisconcerned.Ithasnoeffectontheoutgrowthofthetail.5.5.Oxaloacetate,likeothertricarboxyliccycleintermediates,hasunfavorableeffectsonneuraltubeclosure.Itoftenimprovestheelongationofthetailinlipoicacid-treatedembryos.6.6.Themeaningoftheseresultsisdiscussed.