(Lewis × DA)F1 hybrid rats were injected once or twice with a small, subsaturating dose of DA anti-Lewis posttransplantation alloantiserum. The time course of antialloantibody response was followed over a period of 30 days by using the PAR assay. After a single injection, antialloantibody titers became first detectable on the 2nd day, they increased until the 11th day and reached a plateau between days 11 and 14; thereafter titers slowly declined. A second injection given 2 weeks after the first was followed, without measurable latency, by rapid exponential increase of antialloantibody titers lasting 6 days. The plateau reached 6 to 8 days after the second injection was 500 times higher than that attained after a single injection. Between the 8th and the 16th day after the booster injection titers slowly declined. We conclude that the response measured represents the de novo synthesis of antialloantibody and not a complex of alloantibody and antigen.
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