Experiments were performed to test the hypothesis that two different cells, a radiosensitive antigen reactive cell and a radioresistant precursor of the antibody producing cell collaborate in antibody synthesis. Twenty-four hours following 500 R x-irradiation, experimental recipient group C mice were injected with normal allogeneic spleen cells and sheep red blood cells (SRBC). Control recipients of group A were irradiated similarly and injected with SRBC alone while control recipients of group B were irradiated similarly and injected simultaneously with SRBC and allogeneic spleen cells from heavily irradiated (1000 R) mice. Five to 10 days after antigen injection, the numbers of plaque-forming cells (PFC) of donor and host origin were determined in the spleens of the three groups. The contribution of donor and host PFC was distinguished by the use of anti-histocompatibility sera. On day 5, the spleens of mice in group C contained 26 times as many PFC as did the spleens of mice in group A. Of these, 80% were donor cells and 20% were of host type. Thus more than five times as many host PFC developed in group C than in group A. At least some of the stimulatory effect on host PFC was independent of the normal donor spleen cells because the spleens of control group B contained twice as many PFC as did those of control group A. On days 6 and 7, the PFC in group C decreased, while those in control group A increased, possibly due to alloimmune reactions between donor graft and recipient. The injection of antidonor H-2 serum into group C on day 3 suppressed the development of PFC of both donor and recipient type. Evidently survival of donor cells beyond day 3 was necessary for the enhanced recipient PFC response. It is concluded that existing data establish the importance of cell interactions in the immune response but do not prove the existence of an antigen-sensitive cell capable of transmitting antigen-specific information to the precursor of the antibody-producing cell.
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机译：进行实验以检验两个不同的细胞，即放射敏感性抗原反应性细胞和抗体产生细胞的抗辐射前体在抗体合成中协同作用的假设。在500 R X辐射后二十四小时，向实验性受体C组小鼠注射正常的同种异体脾细胞和绵羊红细胞（SRBC）。相似地照射A组的对照接受者并单独注射SRBC，而相似地照射B组的对照接受者并同时注射来自强辐照（1000 R）小鼠的SRBC和同种异体脾细胞。抗原注射后5至10天，测定三组脾中供体和宿主来源的斑块形成细胞（PFC）的数目。通过使用抗组织相容性血清来区分供体和宿主PFC的贡献。在第5天，C组小鼠脾脏中PFC含量是A组小鼠脾脏的26倍。其中，供体细胞占80％，宿主类型占20％。因此，C组中产生的宿主PFC的数量是A组的五倍多。对宿主PFC的至少某些刺激作用独立于正常供体脾细胞，因为对照组B的脾脏所含PFC数量是对照组的两倍。在第6天和第7天，C组的PFC下降，而对照组A的PFC上升，这可能是由于供体移植物和受体之间的同种免疫反应。在第3天，将抗供体H-2血清注射到C组中，抑制了供体和受体类型的PFC的发展。显然，对于增强的受体PFC应答，超过3天的供体细胞存活是必要的。结论是现有数据确定了细胞相互作用在免疫应答中的重要性，但没有证明能够将抗原特异性信息传递至抗体产生细胞前体的抗原敏感细胞的存在。
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