A solid phase micro-immunoradiometric assay (micro-SPIRA) for the detection of hepatitis e antigen (HBeAg) and antibody has been developed. Chimpanzee anti-HBe/2 was developed by repeated immunizations with purified antigen containing HBeAg/1 and HBeAg/2. An anti-HBe/2 titer of 1:4 was determined by immunodiffusion (ID) analysis. Anti-HBe/1 was not detected. The anti-HBe IgG used in the assay was purified from plasma by a combination of DEAE-cellulose and affinity chromatography. The sensitivity of the micro-SPIRA for antigen and antibody was 193 ng/ml and 65 ng/ml, respectively. By comparing relative endpoint titers obtained by ID to micro-SPIRA, it was determined that micro-SPIRA for antigen and antibody is 320 and greater than 1300 times more sensitive, respectively, than ID. The specificity of the assay was ascertained by the examination of various non-B specimens. The application of the assay to a panel of 50 hepatitis B surface antigen (HBsAg)-positive specimens resulted in an increase in positivity of 18% for antigen and 22% for antibody.
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机译:已经开发了用于检测戊型肝炎抗原(HBeAg)和抗体的固相微免疫放射测定法(micro-SPIRA)。黑猩猩抗HBe / 2是通过用含有HBeAg / 1和HBeAg / 2的纯化抗原重复免疫而开发的。通过免疫扩散(ID)分析确定抗-HBe / 2滴度为1:4。未检测到抗HBe / 1。通过DEAE-纤维素和亲和层析的结合,从血浆中纯化了用于测定的抗-HBe IgG。 micro-SPIRA对抗原和抗体的敏感性分别为193 ng / ml和65 ng / ml。通过将ID获得的相对终点滴定度与micro-SPIRA进行比较,可以确定对抗原和抗体的micro-SPIRA分别比ID高320倍和1300倍以上。通过检查各种非B样本确定测定的特异性。将测定法应用于一组50个乙型肝炎表面抗原(HBsAg)阳性样本后,抗原阳性率增加18%,抗体阳性率增加22%。
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