Two anthranilic acid type AI agents, niflumic acid (Nif) and its benzene analog, flufenamic acid (Flu), were examined for anti-C activity in vitro . The effects of these agents were examined in kinetic assays of the uptake of C1?, C2, C3, and C5 by sensitized red cells (EA) at 25°C with isolated guinea pig C components. Flu was consistently more inhibitory than Nif at comparable concentrations in the test systems studied. Nif, Flu and phlorizin (Pz), each at 9 × 10-4 M, decreased the rates of uptake of C components (site formation) by EA-C complexes as shown in Table I.At 9 × 10-4 M, Flu prevented the activation of C2 by EAC1?4 by 68%. Neither C3 nor C5 activation was affected by the agents. 5 reactivity was decreased by Nif (8%) and Flu (68%) at 9 × 10-4 M at 25°C, while Pz had no effect.![Figure][1]/img [1]: pending:yes
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