Detection of Rabbit Pancreas-Specific Isoantibodies by Bis-Diazotized Benzidine Hemagglutination

摘要

A BDB hemagglutination technique allowed the detection of pancreas-specific isoantibodies in the rabbit. It uses 1% normal rabbit serum plus 0.075 M sucrose as diluent. Hemagglutinins were absent from the serum prior to immunization. Upon injection of rabbit pancreas extract, antibody specific for rabbit pancreas was produced; no reaction was observed with other rabbit organs or with the pancreas of other species. In contrast to the complement fixation tests, cross-reactions with rabbit ileum were not found by BDB hemagglutination. No examples of autoantibodies were encountered.A comparison of isoantigen-isoantibody patterns of rabbit pancreas antisera with individual rabbit pancreas extracts, using both complement fixation and BDB hemagglutination techniques, showed differences in reaction patterns. Reduction and alkylation had no effect on the hemagglutinating capacity of the antiserum, while the complement-fixing activity was abolished.