Complement-fixing antibodies to Mycoplasma pneumoniae were found to be inactivated to a large degree by collection onto filter paper discs, a procedure which is highly effective for the preservation of viral CF antibodies. By means of sucrose density gradient centrifugation, Sephadex gel filtration and mercaptoethanol inactivation it was shown that CF antibodies for Mycoplasma pneumoniae may consist entirely or in part of 19 S γ-globulins. Absorption onto filter paper discs or drying was found to inactivate 19 S CF antibodies, but had no effect on 7 S CF antibodies for Mycoplasma pneumoniae .
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机译:通过收集到滤纸盘上,发现肺炎支原体的补体固定抗体在很大程度上失活,这种方法对于保存病毒CF抗体非常有效。通过蔗糖密度梯度离心,Sephadex凝胶过滤和巯基乙醇灭活,显示肺炎支原体的CF抗体可能全部或部分由19 Sγ-球蛋白组成。发现吸收到滤纸盘上或干燥使19 S CF抗体失活,但对肺炎支原体对7 S CF抗体没有影响。
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