1. 1. Cells which previously have been sensitized by incomplete antibody are susceptible to the action of papain and agglutinate when treated by papain.2. 2. When sensitized cells are mixed with papain-treated cells clumping occurs which involves both treated and sensitized cells. Optimum clumping is observed when the two types of cell are present in equal amounts. It appears that the clumping is due to the linking of treated cells to untreated (previously sensitized) cells by the antibody.3. 3. Carboxypeptidase is able to render cells agglutinable by incomplete antibody. This fact supports the idea that enzyme treatment removes interfering substances which, if present, prevent sensitized cells from clumping.
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