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外文期刊>The journal of immunology
>Functional effects of thymopoietin32-36 (TP5) on cytotoxic lymphocyte precursor units (CLP-U). I. Enhancement of splenic CLP-U in vitro and in vivo after suboptimal antigenic stimulation.
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Functional effects of thymopoietin32-36 (TP5) on cytotoxic lymphocyte precursor units (CLP-U). I. Enhancement of splenic CLP-U in vitro and in vivo after suboptimal antigenic stimulation.
Cytotoxic lymphocyte precursor units (CLP-U) were enumerated in the spleens of C57BL/6 mice 3 days after i.p. injections of synthetic thymopoietin32-36 (TP5). One hundred to 1000 ng TP5/mouse potentiated splenic CLP-U, this effect being detectable only after suboptimal allogeneic sensitization (with 1.2 x 10(5) mitomycin-C treated DBA cells). This elevation of CLP-U persisted in the injected mice for at least 14 days. Control peptide did not affect CLP-U. In vitro incubation of 0.01 to 0.1 ng/ml of TP5 with normal C57BL/6 spleen cells also enhanced CLP-U after suboptimal allogeneic stimulation; high concentrations of TP5 caused suppression of CLP-U and this was detectable with optimal sensitization conditions. Thus TP5, in vitro and in vivo, appears to regulate immune responsiveness and this regulation varies with TP5 dosage and with the immune stimulus.
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机译:腹膜内注射3天后,在C57BL / 6小鼠的脾脏中列举出细胞毒性淋巴细胞前体单位(CLP-U)。注射合成胸腺生成素32-36(TP5)。一百至1000 ng TP5 /小鼠增强的脾脏CLP-U,只有在次优的同种异体敏化(使用1.2 x 10(5)丝裂霉素C处理的DBA细胞)后才能检测到这种效果。 CLP-U的这种升高在注射的小鼠中持续至少14天。对照肽不影响CLP-U。在最佳异体刺激后,将0.01-0.1 ng / ml TP5与正常C57BL / 6脾脏细胞一起体外孵育也会增强CLP-U。高浓度的TP5引起CLP-U抑制,在最佳的敏化条件下可以检测到。因此,TP5在体外和体内似乎调节免疫应答性,并且该调节随TP5剂量和免疫刺激而变化。
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