Fractionation of Splenic Antibody-Forming Cells on Glass Bead Columns

摘要

Rat spleen cell suspensions were separated into two fractions by passage over glass bead columns. The fraction passing through the column in the presence of divalent cations consisted predominantly of small mononuclear cells and was depleted of antibody-synthesizing cells, as measured by the Jerne technique. The fraction eluted from the beads by ethylenediaminetetraacetic acid was enriched in antibody-synthesizing and deoxyribonucleic acid-synthesizing cells, granulocytes and large mononuclear cells. Although the enrichment was small, the results revealed an interesting heterogeneity of splenic mononuclear cells.