pBinding of specific microbial epitopes [MAMPs (microbe-associated molecular patterns)] to PRRs (pattern recognition receptors) and subsequent receptor kinase activation are key steps in plant innate immunity. One of the earliest detectable events after MAMP perception is a rapid and transient rise in cytosolic Casup2+/sup levels. In plants, knowledge about the signalling events leading to Casup2+/sup influx and on the molecular identity of the channels involved is scarce. We used a transgenic iArabidopsis thaliana/i line stably expressing the luminescent aequorin Casup2+/sup biosensor to monitor pharmacological interference with Casup2+/sup signatures following treatment with the bacterial peptide MAMPs flg22 and elf18, and the fungal carbohydrate MAMP chitin. Using a comprehensive set of compounds known to impede Casup2+/sup-transport processes in plants and animals we found strong evidence for a prominent role of amino acid-controlled Casup2+/sup fluxes, probably through iGluR (ionotropic glutamate receptor)-like channels. Interference with amino acid-mediated Casup2+/sup fluxes modulates MAMP-triggered MAPK (mitogen-activated protein kinase) activity and affects MAMP-induced accumulation of defence gene transcripts. We conclude that the initiation of innate immune responses upon flg22, elf18 and chitin recognition involves apoplastic Casup2+/sup influx via iGluR-like channels./p
展开▼
