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外文期刊>The biochemical journal
>Cholesterol-dependent partitioning of PtdIns(4,5)P2 into membrane domains by the N-terminal fragment of NAP-22 (neuronal axonal myristoylated membrane protein of 22 kDa)
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Cholesterol-dependent partitioning of PtdIns(4,5)P2 into membrane domains by the N-terminal fragment of NAP-22 (neuronal axonal myristoylated membrane protein of 22 kDa)
pA myristoylated peptide corresponding to the N-terminus of NAP-22 (neuronal axonal myristoylated membrane protein of 22 kDa) causes the quenching of the fluorescence of BODIPY?-TMR-labelled PtdIns(4,5)iP/isub2/sub in bilayers of 1-palmitoyl-2-oleoyl phosphatidylcholine containing 40 mol% cholesterol and 0.1 mol% BODIPY?–PtdIns(4,5)sub2/sub. Both fluorescence spectroscopy and total internal reflectance fluorescence microscopy revealed the cholesterol-dependent nature of PtdIns(4,5)iP/isub2/sub-enriched membrane-domain formation./p
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机译：>与NAP-22（神经轴突轴突肉豆蔻酰化膜蛋白为22 kDa）的N端相对应的肉豆蔻酰化肽引起BODIPY？-TMR标记的PtdIns（4,5） P < / i> 2 在包含40 mol％胆固醇和0.1 mol％BODIPY？–PtdIns（4,5） 2 的1-棕榈酰基-2-油酰基磷脂酰胆碱的双层中。荧光光谱法和全内反射荧光显微镜法均显示富含PtdIns（4,5） P 2 的膜结构域具有胆固醇依赖性。
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