The gene for human TATA-binding-protein-associated factor (TAFII) 170: structure, promoter and chromosomal localization

摘要

pThe TATA-binding protein (TBP) plays a central role in eukaryotic transcription and forms protein complexes with TBP-associated factors (TAFs). The genes encoding TAFsubII/sub proteins frequently map to chromosomal regions altered in human neoplasias. TAFsubII/sub170 of B-TFIID is a member of the SF2 superfamily of putative helicases. Members of this superfamily have also been implicated in several human genetic disorders. In this study we have isolated human genomic clones encoding iTAFsubII/sub170/i and we show that the gene contains 37 introns. Ribonuclease-protection experiments revealed that iTAFsubII/sub170/i has multiple transcription start sites, consistent with the observation that the promoter lacks a canonical TATA box and initiator element. Deletion analysis of the promoter region showed that a fragment of 264 bp is sufficient to direct transcription. In addition, we determined the chromosomal localization by two independent methods which mapped the gene to human chromosome 10q22-q23 between the markers D10S185 and WI-1183. The region surrounding these markers has been implicated in several human disorders./p