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外文期刊>The biochemical journal
>Arterial oxygen partial pressures reduce the insulin-dependent induction of the perivenously located glucokinase in rat hepatocyte cultures: mimicry of arterial oxygen pressures by H2O2
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Arterial oxygen partial pressures reduce the insulin-dependent induction of the perivenously located glucokinase in rat hepatocyte cultures: mimicry of arterial oxygen pressures by H2O2
pLiver glucokinase (GK) is localized predominantly in the perivenous zone. GK mRNA was induced by insulin maximally under venous Osub2/sub partial pressure (ip/iOsub2/sub) and only half-maximally under arterial ip/isub/subOsub2/sub. CoClsub2/sub and desferrioxamine mimicked venous ip/iOsub2/sub and enhanced the insulin-dependent induction of GK mRNA under arterial ip/iOsub2/sub. Hsub2/subOsub2/sub mimicked arterial ip/iOsub2/sub and reduced insulin-induced GK mRNA under venous ip/iOsub2/sub to the lower arterial levels. Thus the zonal Osub2/sub gradient in liver seems to have a key role in the heterogenous expression of the GK gene./p
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机译：>肝葡萄糖激酶（GK）主要位于静脉区。胰岛素在静脉O 2 分压（ p O 2 ）下最大诱导GK mRNA，而在动脉 p下最大诱导半数 O 2 。 CoCl 2 和去铁草胺模拟静脉 p O 2 ，并增强了动脉 p 下胰岛素依赖性GK mRNA的诱导O 2 。 H 2 O 2 模拟动脉 p O 2 并降低静脉 p下胰岛素诱导的GK mRNA O 2 到达较低的动脉水平。因此，肝脏的O 2 梯度带似乎在GK基因的异源表达中起着关键作用。
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