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>N.m.r. lipid profiles of cells, tissues and body fluids. Neutral, non-acidic and acidic phospholipid analysis of Bond Elut chromatographic fractions
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N.m.r. lipid profiles of cells, tissues and body fluids. Neutral, non-acidic and acidic phospholipid analysis of Bond Elut chromatographic fractions
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机译:N.m.r.细胞,组织和体液的脂质分布。 Bond Elut色谱馏分的中性,非酸性和酸性磷脂分析
pAlthough the advantages of iin vitro/i n.m.r. analysis of cellular lipids have been documented, this purely n.m.r. approach also has drawbacks. Rapid and quantitative separation of total lipids into neutral lipids, non-esterified fatty acids, non-acidic phospholipids and acidic phospholipids using Bond Elut ion-exchange columns as demonstrated here permitted a more quantitative and complete n.m.r. analysis of glycerides, cholesterol, saturated and unsaturated sphingolipids and ether lipids, as well as of diacylcholine and ethanolamine lipids. Acidic lipids were also analysed. The fatty acid compositions of the intact lipids in each of the four Bond Elut fractions were determined from the n.m.r. spectrum of each fraction./p
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