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外文期刊>The biochemical journal
>The cloning and expression of the aroL gene from Escherichia coli K12. Purification and complete amino acid sequence of shikimate kinase II, the aroL-gene product
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The cloning and expression of the aroL gene from Escherichia coli K12. Purification and complete amino acid sequence of shikimate kinase II, the aroL-gene product
pThe aroL gene encoding the enzyme shikimate kinase II was cloned from Escherichia coli K12. Construction of over-expressing strains permitted for the first time the purification to homogeneity of a monofunctional shikimate kinase. The complete amino acid sequence of shikimate kinase II was determined by a combined nucleotide and direct amino acid sequencing strategy. E. coli shikimate kinase II is a monomeric enzyme containing 173 amino acid residues with a calculated Mr 18,937. The amino acid sequence contains a region homologous with other kinases and ATP-requiring enzymes. Evidence is presented suggesting that the transcriptional start site of the aroL gene is located within a potential operator site./p
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机译:从大肠杆菌K12中克隆编码shikimate激酶II的aroL基因。过表达菌株的构建首次允许纯化单功能sh草酸激酶的均质性。 iki草酸激酶II的完整氨基酸序列通过核苷酸和直接氨基酸测序策略的组合来确定。 E. coli shikimate激酶II是一种单体酶,包含173个氨基酸残基,计算得出的Mr为18,937。氨基酸序列包含与其他激酶和需要ATP的酶同源的区域。有证据表明,aroL基因的转录起始位点位于潜在的操纵位点内。 p>
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