pResonance-Raman spectra of Japanese-lacquer-tree (Rhus vernicifera) laccase, type-2-copper-depleted laccase and the latter form treated with H2O2 were measured in liquid and frozen solution, on excitation into the 600 nm absorption band. Significant changes in intensity and/or frequency of the bands lying in the 370-430 cm-1 region were observed on freezing, indicating local structural rearrangements taking place at the blue copper site. These findings corroborate previous suggestions based on e.p.r. measurements and redox data [Morpurgo, Calabrese, Desideri & Rotilio (1981) Biochem. J. 193, 639-642]. They show the strong dependence of the physical properties of blue copper centres on local symmetry. Some conclusions on the origin of the Raman bands are also drawn./p
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机译：在液体和冷冻溶液中，在激发到600 nm吸收带的条件下，测量了日本漆树（Rhus vernicifera）漆酶，贫2型铜漆酶和过氧化氢处理的后者的共振拉曼光谱。 。冷冻时观察到位于370-430 cm-1区域的条带强度和/或频率发生了显着变化，表明在蓝色铜位处发生了局部结构重排。这些发现证实了先前基于e.p.r.测量和氧化还原数据[Morpurgo，Calabrese，Desideri＆amp; Rotilio（1981）生物化学。 J. 193，639-642]。它们显示出蓝铜中心的物理特性强烈依赖于局部对称性。还得出了有关拉曼乐队起源的一些结论。
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