A micro method for simultaneous determination of galactosyltransferase and 5′-nucleotidase activities in cell fractions

摘要

pGalactosyltransferase and 5′-nucleotidase were assayed in the same reaction mixture, with ovalbumin as exogenous acceptor of (14-C)galactose and with (3-H)AMP as the substrate for the 5′-nucleotidase assay. The substrates and reaction products of either assay had no significant effect on the activity of the other enzyme./p