pNuclear acidic proteins isolated from rat brain, heart, kidney and liver showed similar, complex patterns on electrophoresis in sodium dodecyl sulphate–polyacrylamide gels. The contamination of nuclear acidic proteins by nuclear-membrane acidic proteins was found to the extent of 11%. Incorporation of [sup3/supH]acetate into the various nuclear acidic proteins iin vivo/i, which were fractionated by polyacrylamide-gel electrophoresis, differed from tissue to tissue. Hydrolysis of these acetylated nuclear acidic proteins with 6m-HCl at 110°C released 70% of the radioactivity, which indicated that labile acetyl groups had been incorporated into these proteins. Analysis of [sup3/supH]acetate-labelled nuclear acidic proteins revealed two acetylated amino acid residues, iN/isup2/sup-acetylserine and iN/isup2/sup-acetyl-lysine. The significance of the role played by nuclear acidic proteins in relation to gene regulation is discussed./p
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机译:>从大鼠脑,心脏,肾脏和肝脏分离出的核酸性蛋白在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中显示相似,复杂的模式。发现核膜酸性蛋白对核酸性蛋白的污染程度为11%。 [ 3 sup> H]乙酸盐掺入到各种体内体内的核酸性蛋白中,这些蛋白通过聚丙烯酰胺凝胶电泳进行分级分离,各组织之间存在差异。在110°C下用6m-HCl水解这些乙酰化的核酸性蛋白释放出70%的放射性,这表明不稳定的乙酰基已掺入这些蛋白中。分析[ 3 sup> H]乙酸盐标记的核酸性蛋白,发现两个乙酰化氨基酸残基, N i> 2 sup>-乙酰丝氨酸和 N < / i> 2 sup>-乙酰赖氨酸。讨论了核酸性蛋白在基因调控中的作用。 p>
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