The purification and properties of extracellular glycosidases of the cellular slime mould Dictyostelium discoideum

摘要

pThe purification of β-iN/i-acetylhexosaminidase, α-glucosidase, α-mannosidase and β-glucosidase from the spent growth medium of iDictyostelium discoideum/i strain Ax-2 myxamoebae is described. β-iN/i-Acetylhexosaminidase and α-glucosidase were obtained in high yield and as homogeneous preparations whereas the α-mannosidase preparation consisted of two electrophoretically distinct isoenzymes. The physical, chemical and kinetic properties of these enzymes are described./p