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A four-iron ferredoxin from Desulfovibrio desulfuricans (Short Communication)

机译:脱硫脱硫弧菌中的四铁铁氧还蛋白(短文)

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pFerredoxin from iDesulfovibrio desulfuricans/i was isolated, purified and crystallized. It contains four iron atoms and four sulphido or ‘acid-labile’ sulphur atoms for a molecule of 6000 daltons. The absorption spectrum in the u.v.–visible region and the electron-paramagnetic-resonance signals of the reduced protein are similar to those observed for other four-iron ferredoxins. The amino acid composition is different from that of iDesulfovibrio gigas/i ferredoxin. The redox potential of ?0.33V at pH7.0 was determined by dye techniques./p
机译:分离,纯化和结晶了来自 Desulfovibrio desulfuricans 的铁氧还蛋白。它包含四个铁原子和四个硫化物或“酸不稳定”的硫原子,分子数为6000道尔顿。还原蛋白在可见光区的吸收光谱和顺电子顺磁共振信号类似于其他四铁氧化还原蛋白所观察到的光谱。氨基酸组成与吉氏脱硫弧菌铁氧还蛋白不同。用染色技术测定了pH7.0时0.33V的氧化还原电位。

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