pThe rate of differential synthesis of β-galactosidase (αlac) was measured in maximally induced cultures of Escherichia coli B/r with 0.01 M-inducer and 0.01 M-cyclic AMP. The value of αlac decreases with growth rate (60% between 0.67 and 2.1 doublings/h) and after a nutritional shift-up. This decrease is presumed to reflect a decrease in the intracellular concentration of free active RNA polymerase after a shift-up, which implies that the increase in ribosome synthesis after a shift-up is due to an active induction of the ribosomal components./p
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机译:在具有0.01 M诱导剂和0.01 M环AMP的大肠杆菌B / r的最大诱导培养物中,测量了β-半乳糖苷酶(αlac)的差异合成速率。营养增长后,αlac值随生长速率(0.67至2.1加倍/小时之间的60%)降低而降低。推测这种减少反映了上移后细胞内游离活性RNA聚合酶的浓度降低,这表明上移后核糖体合成的增加是由于核糖体成分的主动诱导所致。 p >
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