Isolation and nature of intracellular peptides from a cephalosporin C-producing Cephalosporium sp

摘要

p1. Three peptides containing α-aminoadipic acid and cysteine have been obtained in small amounts from the mycelium of a iCephalosporium/i sp. 2. The peptides were precipitated as cuprous mercaptides together with glutathione and resolved from the latter and from each other by preparative paper electrophoresis and chromatography either in the sulphonic acid form or as iS/i-sulphonyl derivatives. From the iS/i-sulphonyl derivatives they were obtained in the thiol form. 3. One peptide (P3) was shown by amino acid analysis and the mass spectrum of the iNS/i-ethoxycarbonyl derivative of its methyl ester to be δ-(l-α-aminoadipyl)-l-cysteinyl-d-valine. A second peptide (P2) contained α-aminoadipic acid, cysteine, valine and glycine, and the third peptide (P1) contained α-aminoadipic acid, cysteine, β-hydroxyvaline and glycine./p