Competitive inhibition and substrate activity of uridine diphosphate 6-deoxygalactose for Escherichia coli uridine diphosphate galactose 4-epimerase (Short Communication)

摘要

pUDP-6-deoxygalactose inhibits the UDP-galactose 4-epimerase (EC 5.1.3.2) from iEscherichia coli/i in a competitive manner with respect to the substrate UDP-galactose, giving iKsubi/sub/i 1.3×10sup-3/supm. As a substrate for the enzyme, it is transformed into UDP-6-deoxyglucose, although the reaction stops before equilibrium is attained. Possible causes of this behaviour are discussed./p