The subcellular distribution of 32P-labelled phospholipids, 32P-labelled ribonucleic acid and 125I-labelled iodoprotein in pig thyroid slices. Effect in vitro of thyrotrophic hormone and dibutyryl-3′,5′-(cyclic)-adenosine monophosphate

摘要

p1. The incorporation iin vitro/i of [sup32/supP]phosphate into phospholipids and RNA and of [sup125/supI]iodide into protein-bound iodine by pig thyroid slices incubated for up to 6hr. was studied. The subcellular distribution of the labelled products formed after incubation with radioactive precursor in the nuclear, mitochondrial, smooth-microsomal, rough-microsomal and cell-sap fractions was also studied. 2. Pig thyroid slices actively took up [sup32/supP]phosphate from the medium during 6hr. of incubation; the rate of incorporation of sup32/supP into phospholipids was two to five times that into RNA. 3. The uptake of [sup125/supI]iodide by the slices from the medium was rapid for 4hr. of incubation, 6–10% of the label being incorporated into iodoprotein. 4. Much of the sup32/supP-labelled phospholipid accumulated in mitochondria and microsomes, whereas the nuclear fraction contained most of the sup32/supP-labelled RNA. After 2hr. of incubation most of the sup32/supP-labelled cytoplasmic RNA accumulated in the rough-microsomal fraction. The major site of localization of proteinbound sup125/supI was the smooth-microsomal fraction, and gradually increasing amounts appeared in the soluble cytoplasm fraction, suggesting a vectorial discharge of [sup125/supI]iodoprotein (presumably thyroglobulin) from smooth vesicles into the colloid. 5. The addition of 0·1–0·4 unit of thyrotrophic hormone/ml. of incubation medium markedly enhanced the accumulation of sup32/supP-labelled phospholipids in the microsomal fractions and to a much smaller extent that of sup32/supP-labelled RNA without any increase in the total uptake of the label. Almost simultaneously the hormone increased the uptake of [sup125/supI]iodide by the slices and enhanced the accumulation of protein-bound sup125/supI in the smooth-microsomal fraction. 6. As a function of time of incubation, thyrotrophic hormone had a biphasic effect on [sup125/supI]iodide uptake and protein-bound sup125/supI formation, the stimulatory effect being reversed after 4hr. of incubation. 7. 6-iN/i-2′-iO/i-Dibutyryl-3′,5′-(cyclic)-AMP, but not 3′,5′-(cyclic)-AMP or 5′-AMP, mimicked the action of thyrotrophic hormone on iodine uptake as well as on iodination of protein. On the other hand, the mimicry by 6-iN/i-2′-iO/i-dibutyryl-3′,5′-(cyclic)-AMP of the stimulatory effect of thyrotrophic hormone on the formation of labelled thyroid phospholipids and RNA was only an apparent one resulting from an enhanced uptake of [sup32/supP]phosphate. 8. It is concluded that thyrotrophic hormone causes a co-ordinated increase in the formation or accumulation of phospholipids, RNA and iodoprotein associated with the endoplasmic reticulum, and that 6-iN/i-2′-iO/i-dibutyryl-3′,5′-(cyclic)-AMP mimics the more rapid effects of thyrotrophic hormone on transport and metabolic functions of thyroid cells, but does not influence their slower biosynthetic responses to the hormone./p