Postmortem Time and Storage Temperature Affect the Concentrations of Hypoxanthine, other Purines, Pyrimidines, and Nucleosides in Avian and Porcine Vitreous Humor

摘要

An HPLC method was used to determine whether postmortem time and storage temperature affect the concentrations of purines, pyrimidines, and nucleosides in avian and porcine vitreous humor. Inosine, hypoxanthine, xanthine, uric acid, uracil, uridine, and thymine were identified in the vitreous humor of chickens (Gallus domestlcus). Time from death to sample collection (0-192 h) influenced the concentrations of all seven compounds (p p pp < 0.0001). It was concluded that postmortem time and temperature are factors that must be taken into consideration when making comparisons of purines, pyrimidines, and nucleosides between groups and in relating postmortem vitreous humor hypoxanthine concentrations to antemortem hypoxia.