LH and FSH values were studied in 30 healthy boys (CA:11.3-14.6 yrs, BA:10.3-16.2 yrs (TW2), Tanner stage G1-G5). Informed consent was obtained. Blood was sampled at 10′ intervals from 12.00-18.00h and from 24.00-06.00h. An IRMA employing 2 monoclonal antibodies with a sensitivity of LH and FSH of 0.10 IU/l and 0.25 IU/l respectively was used. Testosterone (T) was measured every hour applying a RIA with a sensitivity of 1.0 nmol/1. Results of mean FSH, of mean LH, of mean LH pulse amplitude (pA), of LH pulse number (pN), of mean Nadir Interval in min.(NI) and of mean T are presented as the median per Tanner stage.Conclusion: At the onset of puberty(Gl) LH is undetectable during the day, while during the night LH is secreted in a pulsatile pattern. During puberty LH level, pA and pN increase, the night ahead. In C5 pN decreases, while pA stil 1 increases, presumable as a result of an increased negative feedback action of T. In contrast to LH, diurnal FSH is detectable in Gl, shows a clear increase already in G2 and has no sleep-wake pattern.
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机译：在30名健康男孩（CA：11.3-14.6岁，BA：10.3-16.2岁（TW2），Tanner阶段G1-G5）中研究了LH和FSH值。获得了知情同意。从12.00-18.00h和24.00-06.00h以10'间隔采样血液。使用的IRMA采用2种单克隆抗体，其LH和FSH的敏感性分别为0.10 IU / l和0.25 IU / l。每小时应用RIA灵敏度为1.0 nmol / 1来测量睾丸激素（T）。平均FSH，平均LH，平均LH脉冲幅度（pA），LH脉冲数（pN），平均最低天数间隔（NI）和平均T的结果表示为每个Tanner阶段的中位数。 ：在青春期开始时，LH在白天无法检测到，而在夜间LH以搏动的方式分泌。在青春期LH水平，pA和pN增加，前一天晚上。在C5中，pN降低，而pA stil 1增加，这可能是T负反馈作用增强的结果。与LH相反，G1中可检测到昼夜FSH，G2中已显示明显的日增FSH，并且没有睡眠-唤醒模式。
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