Seven normal adult volunteers (aged between 22 and 60 years) received a purine free diet for eight days (meat, fish, ham, salami and sausages, eggs, legumes, chocolate, wine, beer, superalcoholics, coca-cola, tea and coffee were rigorously excluded; noodles, rice, tomato, bread, cheese and sweets were allowed). Plasma and 24 h urine were analyzed both before and after the experiment for uric acid, hypoxanthine and xanthine content by high performance liquid chromatography (HPLC), as previously described (1). Urinary excretion of uric acid and xanthine was reduced, that of hypoxanthine rose: changes were more evident in plasma, where there was a highly significant rise (P < 0.01) in both oxypurines and lower values for uric acid. Results were surprising in part and interpretation was difficult. Increased purine catabolism, associated with a tendency of the body to spare oxypurines, provoked with a subsequent increase in exchange of oxypurines between tissues: the behavior of xanthine oxidase - which is sensitive to dietary changes and to purine-intake: could partially explain the results.(1) M.Lorenzi et al., Tumori 73, 289–294, 1987.
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