Photooxidation of Parenteral Multivitamins Induces Hepatic Steatosis in a Neonatal Guinea Pig Model of Intravenous Nutrition

摘要

Photooxidation of multivitamin solutions results in the generation of peroxides. Because peroxides are associated with hepatic steatosis and fibrosis as well as cholestasis, we questioned whether multivitamins are implicated in hepatic complications of parenteral nutrition. Guinea pig pups were assigned to groups receiving intravenously either total parenteral nutrition, photo-protected or not, or a control solution (5% dextrose + 0.45% NaCl) supplemented with either a) multivitamins; b) photo-protected multivitamins; c) multivitamins without riboflavin; or d) peroxides (H2O2, tert-butylhydroperoxide). After 4 d, liver was sampled for histology and isoprostane-F2α levels, a marker of radical attack. Multivitamins as well as total parenteral nutrition were associated with steatosis (scored 0–4), the severity of which was reduced (p 2O2 is the major peroxide contaminating multivitamins, it did not induce steatosis scores different than the controls. Compared with controls, hepatic isoprostane-F2α content increased in animals infused with H2O2 (p Abbreviations: C, control; CVI, Cernevit multivitamins; MVP, Multi-12 pediatric multivitamins; MVI, pediatric multivitamins; TBH, tert-butylhydroperoxide; TPN, total parenteral nutrition