337 PCR ANALYSIS FOR DETECTION OF MYCOPLASMA PNEUMONIAE (MP): A USEFUL TEST IN CHILDHOOD LOWER RESPIRATORY TRACT INFECTIONS (LTRI)

摘要

We prospectively studied the role of MP in childhood LTRI. Children aged 3 months to 12 years with a chest X-ray infitrate were included. Excluded were immunodeficiency disorders, aspiration, congenital malformations, nasogastric tube feeding, cystic fibrosis and severe retardation. A complement fixation test and an IF assay measuring IgM and IgG antibodies on day 1 and 14 were performed to confirm infection with MP. A four-fold rise in IgG titers or a positive IgM titer were considered diagnostic. On day 1 a nasopharyngeal aspirate was taken for culture of MP and PCR analysis. The primers used in PCR analysis were: Myc 16S and Myc PL Over a period of 11 months 34 children (15F, 19M) with a mean age of 5.5 years were included. In 10 patients (29%) MP infection was detected. The results of serologic testing(SER.), culture(CULT.) and PCR are shown below:When using SER as a gold standard, sensitivity of CULT. was 30% and specificity was 100%, whereas sensitivity of PCR was 100% and specificity 100%. This shows that PCR may be a useful test in the early diagnosis of childhood LRTI by MP.