ANP immunoreactive protein (RIAtotal) in urine does not represent alpha-h-ANP COD. ANP-99-126 (ANP), but contains at least one other ANP-like peptide, called urodilatin COD ANP-95-126 (URO). The percentages of ANP and URO in urines of different populations are not known. We seperated URO and ANP by HPLC and then determined the concentrations of the 2 fractions by ANP-RIA. We report first results of this technique in 6 healthy neonates (age 2-19 d), 4 infants (age 1-10 mo) and 10 male volunteers (age 19-22 y).RIAtotal concentration was higher (pNA.Conclusion: The percentages of URO and AMP did not differ between newborns, infants and adults. The role of urinary ANP and URO and the nature of the proportion of RIAtotal not found in the ANP- and URO-fractions need further investigation.
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