Objective: To establish an electrochemical immunosensor for the determination of serum trypsin levels using a multiwall carbon nanotubes (MWCNTs)-composite-modified electrode. Method: A MWCNT composite coated on the surface of bare gold electrodes was used for fixation of an anti-trypsin antibody. The assembly process and the performance indicators, including sensitivity, linear range of detection, anti-jamming performance, and stability, of the electrochemical immunosensor were examined by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Results: With optimized experimental conditions, the difference of the current value measured by differential pulse voltammetry (DPV) showed a linear relationship with the concentration of serum trypsin within 0.10–100 ng/mL. The detection limit for trypsin using this sensor was 0.002 ng/mL. Conclusions: The electrochemical immunosensor built using the MWCNT-composite-modified electrode is simple to operate and has a fast response time, along with a wide linear range, high sensitivity, and accuracy, making it suitable for serum trypsin detection.
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机译：目的：建立一种使用多壁碳纳米管（MWCNTs）复合修饰电极测定血清胰蛋白酶水平的电化学免疫传感器。方法：将裸露在金电极表面的MWCNT复合材料用于固定抗胰蛋白酶抗体。通过循环伏安法（CV）和电化学阻抗谱（EIS）检查了电化学免疫传感器的组装过程和性能指标，包括灵敏度，检测的线性范围，抗干扰性能和稳定性。结果：在优化的实验条件下，通过差分脉冲伏安法（DPV）测量的电流值差异与血清胰蛋白酶浓度在0.10–100 ng / mL之间呈线性关系。使用该传感器对胰蛋白酶的检出限为0.002 ng / mL。结论：使用MWCNT复合修饰电极构建的电化学免疫传感器操作简便，响应时间快，线性范围宽，灵敏度高，准确性高，适用于血清胰蛋白酶检测。
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