In this study a novel sensitive nanogold particle sensor enhancement based on mixed self-assembled monolayers was explored and used to construct a Surface Plasmon Resonance (SPR) immunosensor to detect Ischemia Modified Albumin (IMA). Compared with a direct binding SPR assay at a limit of detection (LOD) of 100 ng/L, gold nanoparticles (AuNPs) of 10 nm dramatically improved the LOD of IMA to 10 ng/L. Meanwhile, no interfering substance that may lead to false positive results was identified. These results suggested that the SPR biosensor presented superior properties, and provided a simple label-free strategy to increase assay sensitivity for further acute coronary syndrome (ACS) diagnosis.
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机译：在这项研究中，探索了一种基于混合自组装单分子膜的新型敏感纳米金粒子传感器增强技术，并用于构建表面等离子体共振（SPR）免疫传感器，以检测缺血修饰的白蛋白（IMA）。与检测限（LOD）为100 ng / L的直接结合SPR分析相比，10 nm的金纳米颗粒（AuNPs）将IMA的LOD显着提高到10 ng / L。同时，未发现可能导致假阳性结果的干扰物质。这些结果表明，SPR生物传感器具有优越的性能，并提供了一种简单的无标记策略来提高测定灵敏度，以进一步诊断急性冠脉综合征（ACS）。
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