A disposable amperometric biosensor for commercial use to detect hydrogen peroxide has been developed. The sensor is based on screen-printed carbon paste electrodes modified by electropolymerization of pyrrole with horseradish peroxidase (HRP) entrapped. The facture techniques of fabricating the enzyme electrodes are suitable for mass production and quality control. The biosensor shows a linear amperometric response to H2O2 from 0.1 to 2.0 mM, with a sensitivity of 33.24 µA mM-1 cm-2. Different operational parameters of electropolymerization are evaluated and optimized.
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机译:已经开发出用于商业用途的用于检测过氧化氢的一次性安培生物传感器。该传感器基于丝网印刷的碳糊电极,该碳糊电极是通过吡咯与截留的辣根过氧化物酶(HRP)进行电聚合而修饰的。酶电极的制造技术适用于大规模生产和质量控制。该生物传感器对H 2 sub> O 2 sub>的线性安培响应从0.1到2.0 mM,灵敏度为33.24 µA mM -1 sup> cm < sup> -2 sup>。评估和优化了电聚合的不同操作参数。
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