In this work, a novel amperometric immunosensor based on multi-walled carbon nanotubes-thionine-chitosan (MWCNTs-THI-CHIT) nanocomposite film as electrode modified material was developed for the detection of chlorpyrifos residues. The nanocomposite film was dropped onto a glassy carbon electrode (GCE), and then the anti-chlorpyrifos monoclonal antibody was covalently immobilized onto the surface of MWCNTs-THI-CHIT/GCE using the crosslinking agent glutaraldehyde (GA). The modification procedure was characterized by using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Under the optimized conditions, a linear relationship between the relative change in peak current of different pulse voltammetry (DPV) and the logarithm of chlorpyrifos solution concentration was obtained in the range from 0.1 to 1.0 × 105 ng/mL with a detection limit of 0.046 ng/mL. The proposed chlorpyrifos immunosensor exhibited high reproducibility, stability, and good selectivity and regeneration, making it a potential alternative tool for ultrasensitive detection of chlorpyrifos residues in vegetables and fruits.
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机译:在这项工作中,开发了一种基于多壁碳纳米管-蛋氨酸-壳聚糖(MWCNTs-THI-CHIT)纳米复合膜作为电极修饰材料的新型安培免疫传感器,用于检测毒死rif残留。将纳米复合膜滴到玻璃碳电极(GCE)上,然后使用交联剂戊二醛(GA)将抗毒死rif单克隆抗体共价固定在MWCNTs-THI-CHIT / GCE的表面上。通过使用循环伏安法(CV)和电化学阻抗谱(EIS)来表征修饰过程。在最佳条件下,不同脉冲伏安法(DPV)峰值电流的相对变化与毒死rif溶液浓度的对数之间呈线性关系,范围为0.1至1.0×10 5 sup> ng / mL的检出限为0.046 ng / mL。拟议中的毒死immuno免疫传感器具有较高的重现性,稳定性,良好的选择性和再生性,使其成为超敏感检测蔬菜和水果中毒死rif残留的潜在替代工具。
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