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>A Biosensor Based on Immobilization of Horseradish Peroxidase in Chitosan Matrix Cross-linked with Glyoxal for Amperometric Determination of Hydrogen Peroxide
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A Biosensor Based on Immobilization of Horseradish Peroxidase in Chitosan Matrix Cross-linked with Glyoxal for Amperometric Determination of Hydrogen Peroxide
An amperometric biosensor for hydrogen peroxide (H2O2) was developed via an easy and effective enzyme immobilization method with the “sandwich” configuration: ferrocene-chitosan: HRP: chitosan-glyoxal using a glassy carbon electrode as the basic electrode. In order to prevent the loss of immobilized HRP under optimized conditions, the biosensor surface was cross-linked with glyoxal. Ferrocene was selected and immobilized on the glassy carbon electrode surface as a mediator. The fabrication procedure was systematically optimized to improve the biosensor performance. The biosensor had a fast response of less than 10 s to H2O2, with a linear range of 3.5×10-5 to 1.1×10-3 M, and a detection limit of 8.0×10-6 M based on S/N = 3.
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机译:通过一种简单有效的酶固定方法开发了一种过氧化氢电流传感器(H 2 sub> O 2 sub>),该酶固定方法为“三明治”构型:二茂铁-壳聚糖:HRP:壳聚糖-乙二醛,使用玻璃碳电极作为基本电极。为了防止在最佳条件下固定化HRP的丢失,将生物传感器表面与乙二醛交联。选择二茂铁并将其固定在玻璃碳电极表面作为介体。系统地优化了制造程序,以提高生物传感器的性能。该生物传感器对H 2 sub> O 2 sub>的响应时间小于10 s,线性范围为3.5×10 -5 sup>至1.1 ×10 -3 sup> M,基于S / N = 3的检测极限为8.0×10 -6 sup>M。
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