Water transport in vascular plants provides remarkable opportunities for various engineering applications due to its highly efficient and powerless transportability. Several previous studies were conducted to regulate the biological responses of plants using noninvasive audible or ultrasound waves. However, the control mechanism of acoustic stimuli applied to plants has not been investigated yet. Thus, the practical application of these stimuli to real plants still exhibits technological limitations. This study experimentally investigated the effects of surface acoustic wave (SAW) frequency on plant transpiration to understand the acoustic-activated leaf transpiration and utilize the advantages of SAW. We captured consecutive images of the enhanced water transport in the test plant ( Epipremnum aureum ) by SAW at three different frequencies (10, 15, and 20 MHz). The dye solution at 15 MHz SAW presented the highest intensity value after 40?min of SAW stimulation. The excitation areas for 15 and 20 MHz SAWs were decreased to 42.3% and 22.6%, respectively, compared with that of 10 MHz SAW. The transpiration rates were directly measured to compare water transport enhancement quantitatively when different SAW frequencies were applied to the same plant leaves. The water transport in the leaves was maximized at 15 MHz SAW, regardless of excitation area.
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