Expression OfPantoea Agglomerans Phytase From A Strong Constitutive Promoter In Arabidopsis Thaliana Plants.

摘要

ABSTRACT In this study we report construction of an efficient gene expression system in plants and subsequent characterization of transgenic Arabidopsis thaliana expressing a bacterial paPhyC phytase gene from Pantoea sp. Phytase gene expression is controlled by a strong 35S constitutive promoter from cauliflower mosaic virus. All identified transgenic plants had multiple T-DNA insertions in the genome. Expression of paPhyC phytase mRNA in plant tissue was confirmed by RT-PCR in the second gene.