Identification of centromere regions in chromosomes of a unicellular red alga, Cyanidioschyzon merolae

摘要

Toinvestigatetheevolutionofcentromerearchitectureinplantcells,itisimportanttoidentifycentromereregionsofprimitivealgae,suchasCyanidioschyzonmerolae.Inapreviousgenomeproject,insilicoanalysispredictedanAT‐richregionineachchromosomeasputativecentromereregions.Here,weidentifiedacentromerepositionineachchromosomebyChIP‐on‐chipanalysisusingananti‐CENP‐Aantibody.Theidentifiedcentromereswereoftheregionaltype,about2–3kbinlengthandcontainednoconsensusorrepeatelements.Centromeresinprimitiveeukaryoticplantcellsmayhaveoriginatedfromtheseregionaltypecentromeres...