Cloning of the human IL‐13Rα1 chain and reconstitution with the IL‐4Rα of a functional IL‐4/IL‐13 receptor complex

摘要

>The human homologue of the recently cloned murine IL-13 binding protein (IL-13Rα1) was cloned from a cDNA library derived from the carcinoma cell line CAKI-1. The cloned cDNA encodes a 427 amino acid protein with two consensus patterns characteristic of the hematopoietic cytokine receptor family and a short cytoplasmic tail. The human protein is 74% identical to the murine IL-13Rα1, and 27% identical to the human IL-13Rα2. CHO cells expressing recombinant hIL-13Rα1 specifically bind IL-13 (K _d≈4 nM) but not IL-4. Co-expression of the cloned cDNA with that of IL-4Rα resulted in a receptor complex that displayed high affinity for IL-13 (K _d≈30 pM), and that allowed cross-competition of IL-13 and IL-4. Electrophoretic mobility shift assay showed that IL-13 and IL-4 were able to activate Stat6 in cells expressing both IL-4Rα and IL-13Rα1, while no activation was observed in cells expressing either one or the other alone.