Electrophoretic behavior of d(GGAAAAAAGG)n, d(CCAAAAAACC)n, and (CCAAAAAAGG)n, and implications for a DNA bending model

摘要

Double stranded multimers and (C₂A₆C₂)n,(C₂A₆G₂)n, and (C₂A₆G₂)n, were prepared from chemically synthesized oligonucleotldes to study the influence of sequences flanking the A_n tract on the curvature of DNA. All these duplexes, including polypurine . polypyrimidine one, exhibit strong retardation in polyacrylamide gel which is indicative of pronounced DNA curvature. ft has been proposed previously that among the bends at the boundary with the ollgo(A) tract two types should be distinguished: 5′-bends and 3′-bends (Koo et al., 1986) This distinction was deduced from different relative mobilities of two specially designed sequences having phased 5′-bends and 3′-bends, respectively. Our data indicate that the substitutions of nucleotides at both 5′ and 3′ boundaries of A₆ tract result in comparable changes in relative mobility. Therefore, for B-B′ bends it is important to take Into account not only whether they are at the 5′ or 3′ end of an ollgo(dA) tract, but also the particular sequences at the boundaries of this tract.