The nucleotide sequence preceding an RNA polymerase initiation site on SV40 DNA. Rart 2. The sequence of the early strand transcript

摘要

The nucleotide sequence of the RNA transcript from the “early” (E) strand of SV40 DNA immediately preceding the preferred E. coll RNA polymerase start site is G-(A-A-A-C, -A-U-)-A-A-A-A-U-G-A-A-U-G-C-A-A-U-U-G-U-U-G-U-U-G-U-U-A-A-C-U-U-G-U-U-U-A-U-U-G-C-A-G-C-U-U-A-U-A-A-U-G-G-U-U-A-C-Ap. The last nucleotide of the sequence is the first nucleotide transcribed by E. coli RNA polymerase from the “E” strand. The DNA template contains a palindrome of 17 residues that includes the Hemophllus Influenza restriction endonuclease cleavage site G-T-T-A-A-Cp. The DNA which gives this transcript lies very close to one end of SV40 DNA segment In the Adeno-SV40 hybrid virus Ad2+ND3 and appears to contain sufficient untranscrlbed Information to specify the E. coll RNA polymerase start.