Inhibition of prostasin-induced ENaC activities by PN-1 and regulation of PN-1 expression by TGF-|[beta]|1 and aldosterone

摘要

Prostasin has been shown to regulate sodium handling in the kidney. Recently, a serine protease inhibitor, protease nexin-1 (PN-1), was identified as an endogenous inhibitor for prostasin. Therefore, we hypothesized that PN-1 may regulate sodium reabsorption by reducing prostasin activity, and that expression of PN-1 was regulated by transforming growth factor-1 (TGF-1) or aldosterone, like prostasin. cRNAs for epithelial sodium channel (ENaC), prostasin, and PN-1 were expressed in Xenopus oocytes, and the amiloride-sensitive sodium currents (INa) were measured. The effect of TGF-1 and aldosterone on the mRNA and protein abundance of PN-1 and ENaC was detected by real-time polymerase chain reaction and immunoblotting in M-1 cells. Expression of PN-1 substantially decreased prostasin-induced INa by approximately 68% in oocytes. Treatment of M-1 cells with 20ng/ml TGF-1 significantly increased protein expression of PN-1 by 3.80.5-fold, whereas administration of 10-6M aldosterone markedly decreased protein expression of PN-1 to 53.76.7%. Basolateral, but not apical, application of TGF-1 significantly reduced Ieq. To elucidate the involvement of PN-1 in basal ENaC activity, we silenced the expression of PN-1 by using short-interfering RNA. This increased Ieq by 1.60.1-fold. Our study indicates that PN-1 could have a natriuretic role by inhibiting prostasin activity and suggests the possibility that aldosterone and TGF- reciprocally regulate the expression of PN-1 in renal epithelial cells contributing to salt retention or natriuresis, respectively by an additional mechanism. PN-1 could represent a new factor that contributes to regulation of ENaC activity in the kidney.