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Structural Characterization and Antifungal Studies of Zinc-Doped Hydroxyapatite Coatings

机译:锌掺杂羟基磷灰石涂层的结构表征和抗真菌研究

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The present study is focused on the synthesis, characterization and antifungal evaluation of zinc-doped hydroxyapatite (Zn:HAp) coatings. The Zn:HAp coatings were deposited on a pure Si (Zn:HAp_Si) and Ti (Zn:HAp_Ti) substrate by a sol-gel dip coating method using a zinc-doped hydroxyapatite nanogel. The nature of the crystal phase was determined by X-ray diffraction (XRD). The crystalline phase of the prepared Zn:HAp composite was assigned to hexagonal hydroxyapatite in the P6 3/m space group. The colloidal properties of the resulting Zn:HAp (x Zn = 0.1) nanogel were analyzed by Dynamic Light Scattering (DLS) and zeta potential. Scanning Electron Microscopy (SEM) was used to investigate the morphology of the zinc-doped hydroxyapatite (Zn:HAp) nanogel composite and Zn:HAp coatings. The elements Ca, P, O and Zn were found in the Zn:HAp composite. According to the EDX results, the degree of Zn substitution in the structure of Zn:HAp composite was 1.67 wt%. Moreover, the antifungal activity of Zn:HAp_Si and Zn:HAp_Ti against Candida albicans ( C. albicans ) was evaluated. A decrease in the number of surviving cells was not observed under dark conditions, whereas under daylight and UV light illumination a major decrease in the number of surviving cells was observed.
机译:本研究的重点是掺杂锌的羟基磷灰石(Zn:HAp)涂层的合成,表征和抗真菌评估。通过使用锌掺杂的羟基磷灰石纳米凝胶的溶胶-凝胶浸涂方法,将Zn:HAp涂层沉积在纯Si(Zn:HAp_Si)和Ti(Zn:HAp_Ti)基底上。晶相的性质通过X射线衍射(XRD)确定。所制备的Zn:HAp复合物的结晶相分配给P6 3 / m空间群中的六角形羟基磷灰石。通过动态光散射(DLS)和Zeta电位分析了所得Zn:HAp(x Zn = 0.1)纳米凝胶的胶体性质。扫描电子显微镜(SEM)用于研究掺杂锌的羟基磷灰石(Zn:HAp)纳米凝胶复合材料和Zn:HAp涂层的形貌。在Zn:HAp复合物中发现了Ca,P,O和Zn元素。根据EDX结果,Zn:HAp复合材料的结构中Zn的取代度为1.67重量%。此外,评价了Zn:HAp_Si和Zn:HAp_Ti对白色念珠菌(C. albicans)的抗真菌活性。在黑暗条件下未观察到存活细胞数量的减少,而在日光和紫外光照射下观察到存活细胞数量的主要减少。

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