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首页> 外文期刊>MATEC Web of Conferences >Kinetic Study on Flooded Soil Recovery Using Soil Containing Arbuscular Mycorrhizal Fungi
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Kinetic Study on Flooded Soil Recovery Using Soil Containing Arbuscular Mycorrhizal Fungi

机译:丛枝菌根真菌在土壤水淹恢复中的动力学研究

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The purpose of this research was to determine the kinetic parameters for flooded soil recovery via soil containing Arbuscular Mycorrhizal fungi (AMF). The general procedures of this experiment started by preparation of simulated flooded soil (FS) and soil containing AMF (SA). Mixed soil was prepared by mixing FS and SA with ratio 1:1. Onion plant was chosen as a host plant and planted in the mixed soil for 14 days. The plantation was conducted in ambient temperature. The nutrients (nitrogen, phosphorus and potassium) concentrations in the soil were tested using HACH Spectrophotomer. The Michaelis-Menten equation was used to study the nutrients recovery in soil. The Lineweaver-Bulk plot was used to solve the Michaelis-Menten equation. From the experiment conducted, the maximum nutrient uptake (Vmax) and bonding affinity (Km) obtained for nitrogen (N) were 6.28mg/l.d and 82.17 mg/l, for phosphorus (P) were 9.80 mg/l.d and 60.96 mg/l.d and for potassium (K) were 0.07mg/l.d and 4.55mg/l. By comparing the result with other researcher, it showed that the Vmax and Km of nitrogen (N) and phosphorus (P) obtained were higher than other research. This was because the onion required a high level of N and P in the soil compared to other host plant.
机译:这项研究的目的是确定通过含丛枝菌根真菌(AMF)的土壤来淹没土壤的动力学参数。该实验的一般程序从准备模拟淹没土壤(FS)和含AMF的土壤(SA)开始。通过以1:1的比例混合FS和SA制备混合土壤。选择洋葱植物作为寄主植物,并在混合土壤中种植14天。种植在环境温度下进行。使用HACH分光光度计测试土壤中的养分(氮,磷和钾)浓度。 Michaelis-Menten方程用于研究土壤中的养分回收。使用Lineweaver-Bulk图来求解Michaelis-Menten方程。根据所进行的实验,获得的最大养分吸收量(Vmax)和对氮(N)的键合亲和力(Km)为6.28mg / ld和82.17 mg / l,对磷(P)为9.80 mg / ld和60.96 mg / ld而钾(K)分别为0.07mg / ld和4.55mg / l。通过与其他研究人员进行比较,结果表明,获得的氮(N)和磷(P)的Vmax和Km均高于其他研究。这是因为与其他寄主植物相比,洋葱在土壤中需要高含量的N和P。

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