Multiple Nucleotide Change in the rpoB gene of Mycobacterium tuberculosi Isolates Correlate with High-level of Resistance to Rifampicin in Belarusian Patients with Active Pulmonary

摘要

The aim of this study was to investigate the significance of multiple-mutations in the rpoB gene, predominant nucleotide changes and its correlation with high levels of resistance to rifampicin in Mycobacterium tuberculosis isolates that were randomly collected from sputa of 44 patients with primary and secondary active pulmonary tuberculosis from different region of Belarus. Drug susceptibility testing was determined using the CDC standard conventional proportional method. DNA extraction, rpoB gene amplification, and DNA sequencing analysis were performed.