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首页> 外文期刊>Infection and immunity >Lung phagocyte bactericidal function in strains of mice resistant and susceptible to Pseudomonas aeruginosa.
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Lung phagocyte bactericidal function in strains of mice resistant and susceptible to Pseudomonas aeruginosa.

机译:对铜绿假单胞菌有抗药性和敏感性的小鼠品系中的肺吞噬细胞杀菌功能。

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The host response to Pseudomonas aeruginosa lung infection varies among inbred mouse strains. Mice of the BALB/c strain are resistant to P. aeruginosa lung infection, whereas mice of the DBA/2 strain are susceptible. This phenotypic variation correlates with a difference in the magnitude of the inflammatory response induced early following infection. In order to determine whether the ability of lung phagocytic cells to kill P. aeruginosa plays a role in the host response to the infection, we measured the in vitro bactericidal activity of resident and inflammatory alveolar and interstitial macrophages, using a temperature-sensitive mutant of P. aeruginosa. Lung macrophages obtained from resistant and susceptible animals displayed similar bactericidal activities, suggesting that the ability of phagocytes to kill P. aeruginosa does not play a crucial role in the outcome of infection. The bactericidal activity of lung phagocytes was also assessed in vivo following endobronchial infection with the temperature-sensitive mutant of P. aeruginosa. Resistant mice showed a rapid influx of polymorphonuclear leukocytes (PMNs) to the bronchoalveolar space which was shortly followed by an efficient clearance of the bacteria. Susceptible mice had a delay in both the inflammatory response to P. aeruginosa and the initiation of bacterial clearance. Susceptible mice have been shown to have a defect in tumor necrosis factor alpha production when infected intratracheally with P. aeruginosa. Intratracheal instillation of tumor necrosis factor alpha to susceptible mice at the time of infection significantly improved the recruitment of PMNs to the site of infection without affecting the process of bacterial clearance. Overall, these results suggest that both recruitment of a high number of PMNs to the lungs and an efficient activation process of the phagocytes are crucial for the prompt clearance of P. aeruginosa.
机译:宿主对铜绿假单胞菌肺部感染的反应因近交小鼠品系而异。 BALB / c株的小鼠对铜绿假单胞菌肺部感染具有抗性,而DBA / 2株的小鼠易感。这种表型变化与感染后早期诱发的炎症反应的大小不同有关。为了确定肺吞噬细胞杀死铜绿假单胞菌的能力是否在宿主对感染的反应中起作用,我们使用了对温度敏感的突变体,测定了居民和炎性肺泡和间质巨噬细胞的体外杀菌活性。铜绿假单胞菌。从抗药性和易感动物获得的肺巨噬细胞显示出相似的杀菌活性,这表明吞噬细胞杀死铜绿假单胞菌的能力在感染结果中没有关键作用。还用铜绿假单胞菌的温度敏感突变体在支气管内感染后在体内评估了肺吞噬细胞的杀菌活性。抗性小鼠显示多形核白细胞(PMN)快速流入支气管肺泡间隙,随后很快清除细菌。易感小鼠对铜绿假单胞菌的炎症反应和细菌清除的启动均具有延迟。当气管内感染铜绿假单胞菌时,易感小鼠已显示出肿瘤坏死因子α产生缺陷。在感染时向易感小鼠气管内滴注肿瘤坏死因子α可显着改善PMN在​​感染部位的募集,而不会影响细菌清除的过程。总体而言,这些结果表明,大量PMN募集到肺部以及吞噬细胞的有效活化过程对于迅速清除铜绿假单胞菌至关重要。

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